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SERPINE1 Chemi-Luminescent ELISA Kit (Rat)

ArtNr OKCD03849
Hersteller AVIVA Systems Biology
Menge 96 Wells
Kategorie
Typ Elisa-Kit
Applikationen CLIA, IA
Specific against Rat (Rattus norvegicus)
Sensitivity 0.312 ng/mL
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias endothelial plasminogen activator inhibitor;PAI;Pai1;PAI-1;PAI1A;Pai1aa;Planh;plasminogen activator inhibitor 1;RATPAI1A;serine (or cysteine) peptidase inhibitor,clade E,member 1;serine (or cysteine) proteinase inhibitor,clade E (nexin,plasminogen activator inhibitor type 1),member 1;serine (or cysteine) proteinase inhibitor,clade E,member 1;serine (or cysteine) proteinase inhibitor,member 1;serpin E1;serpin peptidase inhibitor,clade E (nexin,plasminogen activator inhibitor type 1),member 1.
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
ELISA Kit
Manufacturer - Category
Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature
Wet Ice
Gene symbol
Serpine1
Gene Fullname
serpin family E member 1
Reconstitution and storage
2°C to 8°C|-20°C
Description of target
Serine protease inhibitor. Inhibits TMPRSS7. Is a primary inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU). As PLAT inhibitor, it is required for fibrinolysis down-regulation and is responsible for the controlled degradation of blood clots. As PLAU inhibitor, it is involved in the regulation of cell adhesion and spreading. Acts as a regulator of cell migration, independently of its role as protease inhibitor. It is required for stimulation of keratinocyte migration during cutaneous injury repair. It is involved in cellular and replicative senescence (By similarity). Plays a role in alveolar type 2 cells senescence in the lung (By similarity). Is involved in the regulation of cementogenic differentiation of periodontal ligament stem cells, and regulates odontoblast differentiation and dentin formation during odontogenesis (By similarity).
Nucleotide accession_num
NM_012620
Protein accession_num
NP_036752
Protein name
Plasminogen activator inhibitor 1
Assay info
Assay Methodology: Quantitative Sandwich ELISA
Kit Components
Component: Amount
Serpine1 Microplate: 96 Wells (12 x 8 Well strips)
Serpine1 Lyophilized Standard: 2
100X Serpine1 Biotinylated Detector Anitbody: 1 x 120 uL
100X Avidin-HRP Conjugate: 1 x 120 uL
Standard Diluent: 1 x 20 mL
Detector Antibody Diluent: 1 x 12 mL
Conjugate Diluent: 1 x 12 mL
30X Wash Buffer: 1 x 20 mL
100X Luminol Substrate: 1 x 2 mL
Substrate Diluent: 1 x 20 mL
Kit detection
Chemiluminescent
Kit duration
2h, 40min
Kit linearity
Sample - 1:2 - 1:4 - 1:8 - 1:16
sodium citrate plasma(n=5) - 80-102% - 97-105% - 99-105% - 82-102%
- - - -
- - - -
Kit principle
The microplate provided in this kit has been pre-coated with an antibody specific to Plasminogen Activator Inhibitor 1 (PAI1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Plasminogen Activator Inhibitor 1 (PAI1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Plasminogen Activator Inhibitor 1 (PAI1) level in the sample or standard.
Kit recovery
Matrix - Recovery range (%) - Average(%)
sodium citrate plasma(n=5) - 96-103 - 99
- -
- -
Kit reproducibility
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Plasminogen Activator Inhibitor 1 (PAI1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Plasminogen Activator Inhibitor 1 (PAI1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type
Plasma

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96 Wells
Lieferbar: In stock
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