PAK4 Chemi-Luminescent ELISA Kit (Human)

ELISA Kit

Wet Ice

p21 (RAC1) activated kinase 4

Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, growth, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates LIMK1, a kinase that also inhibits the activity of cofilin. Phosphorylates integrin beta5/ITGB5 and thus regulates cell motility. Phosphorylates ARHGEF2 and activates the downstream target RHOA that plays a role in the regulation of assembly of focal adhesions and actin stress fibers. Stimulates cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Alternatively, inhibits apoptosis by preventing caspase-8 binding to death domain receptors in a kinase independent manner. Plays a role in cell-cycle progression by controlling levels of the cell-cycle regulatory protein CDKN1A and by phosphorylating RAN.

NP_001014831

Assay Methodology: Quantitative Sandwich ELISA

Chemiluminescent

Sample - 1:2 - 1:4 - 1:8 - 1:16
Serum (n=5) - 86-101% - 79-99% - 84-95% - 91-98%
EDTA Plasma (n=5) - 91-102% - 80-101% - 97-104% - 96-105%
Heparin Plasma (n=5) - 97-105% - 79-98% - 92-105% - 78-91%

Matrix - Recovery range (%) - Average(%)
Serum (n=5) - 98-105 - 101
EDTA Plasma (n=5) - 80-91 - 85
Heparin Plasma (n=5) - 78-104 - 98

Serum, plasma and other biological fluids