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SERPINA5 Chemi-Luminescent ELISA Kit (Human)

SERPINA5 Chemi-Luminescent ELISA Kit (Human)

ArtNr	OKCD03858
Hersteller	AVIVA Systems Biology
Menge	96 Wells
Kategorie	ELISA ELISA & Immunoassays ELISA Kits Immunoassay CLIA
Typ	Elisa-Kit
Applikationen	CLIA, IA
Specific against	Human (Homo sapiens)
Sensitivity	1.25 ng/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	acrosomal serine protease inhibitor;PAI3;PAI-3;PCI;PCI-B;PLANH3;plasma serine protease inhibitor;Plasminogen activator inhibitor 3;plasminogen activator inhibitor III;plasminogen activator inhibitor-3;PROCI;protein C inhibitor;serine (or cysteine) proteinase inhibitor,clade A (alpha-1 antiproteinase,antitrypsin),member 5;Serpin A5;serpin peptidase inhibitor,clade A (alpha-1 antiproteinase,antitrypsin),member 5.
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Type	ELISA Kit
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	SERPINA5
Gene Fullname	serpin family A member 5
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	Heparin-dependent serine protease inhibitor acting in body fluids and secretions. Inactivates serine proteases by binding irreversibly to their serine activation site. Involved in the regulation of intravascular and extravascular proteolytic activities. Plays hemostatic roles in the blood plasma. Acts as a procoagulant and proinflammatory factor by inhibiting the anticoagulant activated protein C factor as well as the generation of activated protein C factor by the thrombin/thrombomodulin complex. Acts as an anticoagulant factor by inhibiting blood coagulation factors like prothrombin, factor XI, factor Xa, plasma kallikrein and fibrinolytic enzymes such as tissue- and urinary-type plasminogen activators. In seminal plasma, inactivates several serine proteases implicated in the reproductive system. Inhibits the serpin acrosin; indirectly protects component of the male genital tract from being degraded by excessive released acrosin. Inhibits tissue-and urinary-type plasminogen activator, prostate-specific antigen and kallikrein activities; has a control on the sperm motility and fertilization. Inhibits the activated protein C-catalyzed degradation of SEMG1 and SEMG2; regulates the degradation of semenogelin during the process of transfer of spermatozoa from the male reproductive tract into the female tract. In urine, inhibits urinary-type plasminogen activator and kallikrein activities. Inactivates membrane-anchored serine proteases activities such as MPRSS7 and TMPRSS11E. Inhibits urinary-type plasminogen activator-dependent tumor cell invasion and metastasis. May also play a non-inhibitory role in seminal plasma and urine as a hydrophobic hormone carrier by its binding to retinoic acid.
Nucleotide accession_num	NM_000624
Protein accession_num	NP_000615
Protein name	Plasma serine protease inhibitor
Assay info	Assay Methodology: Quantitative Sandwich ELISA
Kit Components	Component: Amount SERPINA5 Microplate: 96 Wells (12 x 8 Well strips) SERPINA5 Lyophilized Standard: 2 100X SERPINA5 Biotinylated Detector Anitbody: 1 x 120 uL 100X Avidin-HRP Conjugate: 1 x 120 uL Standard Diluent: 1 x 20 mL Detector Antibody Diluent: 1 x 12 mL Conjugate Diluent: 1 x 12 mL 30X Wash Buffer: 1 x 20 mL 100X Luminol Substrate: 1 x 2 mL Substrate Diluent: 1 x 20 mL
Kit detection	Chemiluminescent
Kit duration	2h, 40min
Kit linearity	Sample - 1:2 - 1:4 - 1:8 - 1:16 sodium citrate plasma(n=5) - 91-104% - 81-101% - 78-99% - 78-98% - - - - - - - -
Kit principle	The microplate provided in this kit has been pre-coated with an antibody specific to Protein C Inhibitor (PCI). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Protein C Inhibitor (PCI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Protein C Inhibitor (PCI) level in the sample or standard.
Kit recovery	Matrix - Recovery range (%) - Average(%) sodium citrate plasma(n=5) - 87-96 - 91 - - - -
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein C Inhibitor (PCI) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein C Inhibitor (PCI) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Plasma

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OKCD03858.pdf

OKCD03858-datasheet.pdf

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Menge: 96 Wells

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