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PDIA2 Chemi-Luminescent ELISA Kit (Human)

PDIA2 Chemi-Luminescent ELISA Kit (Human)

ArtNr	OKCD03872
Hersteller	AVIVA Systems Biology
Menge	96 Wells
Kategorie	ELISA ELISA & Immunoassays ELISA Kits Immunoassay CLIA
Typ	Elisa-Kit
Applikationen	CLIA, IA
Specific against	Human (Homo sapiens)
Sensitivity	0.156 ng/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	pancreas-specific protein disulfide isomerase;pancreatic protein disulfide isomerase;PDA2;PDI;PDIP;PDIR;protein disulfide isomerase,pancreatic;protein disulfide isomerase-associated 2;protein disulfide-isomerase A2;Rho GDP dissociation inhibitor gamma.
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Type	ELISA Kit
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	PDIA2
Gene Fullname	protein disulfide isomerase family A member 2
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	Acts as an intracellular estrogen-binding protein. May be involved in modulating cellular levels and biological functions of estrogens in the pancreas. May act as a chaperone that inhibits aggregation of misfolded proteins.
Nucleotide accession_num	NM_006849
Protein accession_num	NP_006840
Protein name	Protein disulfide-isomerase A2
Assay info	Assay Methodology: Quantitative Sandwich ELISA
Kit Components	Component: Amount PDIA2 Microplate: 96 Wells (12 x 8 Well strips) PDIA2 Lyophilized Standard: 2 100X PDIA2 Biotinylated Detector Anitbody: 1 x 120 uL 100X Avidin-HRP Conjugate: 1 x 120 uL Standard Diluent: 1 x 20 mL Detector Antibody Diluent: 1 x 12 mL Conjugate Diluent: 1 x 12 mL 30X Wash Buffer: 1 x 20 mL 100X Luminol Substrate: 1 x 2 mL Substrate Diluent: 1 x 20 mL
Kit detection	Chemiluminescent
Kit duration	2h, 40min
Kit principle	The microplate provided in this kit has been pre-coated with an antibody specific to Protein Disulfide Isomerase (PDI). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Protein Disulfide Isomerase (PDI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Protein Disulfide Isomerase (PDI) level in the sample or standard.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Disulfide Isomerase (PDI) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Disulfide Isomerase (PDI) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Tissue homogenates and other biological fluids

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

OKCD03872.pdf

OKCD03872-datasheet.pdf

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Einfache Bestellung Periodische Bestellung

Menge: 96 Wells

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Lieferung vsl. bis 11.12.2025

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