PIM3 Chemi-Luminescent ELISA Kit (Human)

ELISA Kit

Wet Ice

Pim-3 proto-oncogene, serine/threonine kinase

Proto-oncogene with serine/threonine kinase activity that can prevent apoptosis, promote cell survival and protein translation. May contribute to tumorigenesis through: the delivery of survival signaling through phosphorylation of BAD which induces release of the anti-apoptotic protein Bcl-X(L), the regulation of cell cycle progression, protein synthesis and by regulation of MYC transcriptional activity. Additionally to this role on tumorigenesis, can also negatively regulate insulin secretion by inhibiting the activation of MAPK1/3 (ERK1/2), through SOCS6. Involved also in the control of energy metabolism and regulation of AMPK activity in modulating MYC and PPARGC1A protein levels and cell growth.

NP_001001852

Assay Methodology: Quantitative Sandwich ELISA

Chemiluminescent

The microplate provided in this kit has been pre-coated with an antibody specific to Pim-3 Oncogene (PIM3). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Pim-3 Oncogene (PIM3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Pim-3 Oncogene (PIM3) level in the sample or standard.

Tissue homogenates and other biological fluids