KITL Chemi-Luminescent ELISA Kit (Mouse)

ELISA Kit

Wet Ice

kit ligand

Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1, 4, 5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.

NP_001334085

Assay Methodology: Quantitative Sandwich ELISA

Chemiluminescent

Sample - 1:2 - 1:4 - 1:8 - 1:16
Serum (n=5) - 97-105% - 81-93% - 86-94% - 98-105%
EDTA Plasma (n=5) - 78-97% - 99-105% - 80-103% - 80-96%
Heparin Plasma (n=5) - 87-98% - 93-103% - 84-91% - 92-101%

Matrix - Recovery range (%) - Average(%)
Serum (n=5) - 79-91 - 85
EDTA Plasma (n=5) - 88-103 - 91
Heparin Plasma (n=5) - 92-105 - 97

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids