MCAM Chemi-Luminescent ELISA Kit (Mouse)

Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit

Mcam

2°C to 8°C|-20°C

NM_023061

Cell surface glycoprotein MUC18

Component: Amount
Mcam Microplate: 96 Wells (12 x 8 Well strips)
Mcam Lyophilized Standard: 2
100X Mcam Biotinylated Detector Anitbody: 1 x 120 uL
100X Avidin-HRP Conjugate: 1 x 120 uL
Standard Diluent: 1 x 20 mL
Detector Antibody Diluent: 1 x 12 mL
Conjugate Diluent: 1 x 12 mL
30X Wash Buffer: 1 x 20 mL
100X Luminol Substrate: 1 x 2 mL
Substrate Diluent: 1 x 20 mL

2h, 40min

The microplate provided in this kit has been pre-coated with an antibody specific to Cluster Of Differentiation 146 (CD146). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Cluster Of Differentiation 146 (CD146). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Cluster Of Differentiation 146 (CD146) level in the sample or standard.

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cluster Of Differentiation 146 (CD146) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cluster Of Differentiation 146 (CD146) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%