TLR4 Chemi-Luminescent ELISA Kit (Mouse)

ELISA Kit

Wet Ice

toll-like receptor 4

Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed:9851930, PubMed:9989976, PubMed:20133493). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:24380872). Also involved in LPS-independent inflammatory responses triggered by free fatty acids, such as palmitate. In complex with TLR6, promotes sterile inflammation in monocytes/macrophages in response to oxidized low-density lipoprotein (oxLDL) or amyloid-beta 42. In this context, the initial signal is provided by oxLDL- or amyloid-beta 42-binding to CD36. This event induces the formation of a heterodimer of TLR4 and TLR6, which is rapidly internalized and triggers inflammatory response, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion. Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-) (By similarity).

NP_067272

Assay Methodology: Quantitative Sandwich ELISA

Chemiluminescent

Sample - 1:2 - 1:4 - 1:8 - 1:16
Serum (n=5) - 86-101% - 79-91% - 91-101% - 92-101%
EDTA Plasma (n=5) - 86-95% - 95-102% - 91-99% - 90-104%
Heparin Plasma (n=5) - 94-102% - 80-101% - 93-101% - 92-104%

Matrix - Recovery range (%) - Average(%)
Serum (n=5) - 92-99 - 96
EDTA Plasma (n=5) - 79-93 - 88
Heparin Plasma (n=5) - 94-105 - 99

Serum, plasma, tissue homogenates and other biological fluids