TXN Chemi-Luminescent ELISA Kit (Human)

ELISA Kit

Wet Ice

thioredoxin

Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.

NP_001231867

Assay Methodology: Quantitative Sandwich ELISA

Chemiluminescent

Sample - 1:2 - 1:4 - 1:8 - 1:16
Serum (n=5) - 81-103% - 91-98% - 83-99% - 80-91%
EDTA Plasma (n=5) - 90-101% - 84-101% - 86-96% - 98-105%
Heparin Plasma (n=5) - 98-105% - 82-99% - 90-102% - 86-101%

Matrix - Recovery range (%) - Average(%)
Serum (n=5) - 83-90 - 87
EDTA Plasma (n=5) - 82-93 - 88
Heparin Plasma (n=5) - 90-98 - 93

Serum, plasma and other biological fluids