CD63 Chemi-Luminescent ELISA Kit (Human)

ELISA Kit

Wet Ice

CD63 molecule

Functions as cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli.

NP_001244318

Assay Methodology: Quantitative Sandwich ELISA

Chemiluminescent

The microplate provided in this kit has been pre-coated with an antibody specific to Tetraspanin 30Cluster of Differentiation 63 (CD63). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Tetraspanin 30Cluster of Differentiation 63 (CD63). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Tetraspanin 30Cluster of Differentiation 63 (CD63) level in the sample or standard.

Tissue homogenates, cell lysates and other biological fluids