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WFS1 Chemi-Luminescent ELISA Kit (Rat)

WFS1 Chemi-Luminescent ELISA Kit (Rat)

ArtNr	OKCD04028
Hersteller	AVIVA Systems Biology
Menge	96 Wells
Kategorie	Metabolism Diabetes ELISA ELISA & Immunoassays ELISA Kits Immunoassay CLIA
Typ	Elisa-Kit
Applikationen	CLIA, IA
Specific against	Rat (Rattus norvegicus)
Sensitivity	0.156 ng/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	Wolfram syndrome 1 (wolframin);Wolfram syndrome 1 homolog;wolframin.
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Type	ELISA Kit
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	Wfs1
Gene Fullname	wolframin ER transmembrane glycoprotein
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	WFS1
Nucleotide accession_num	NM_031823
Protein accession_num	NP_114011
Protein name	WFS1
Assay info	Assay Methodology: Quantitative Sandwich ELISA
Kit Components	Component: Amount Wfs1 Microplate: 96 Wells (12 x 8 Well strips) Wfs1 Lyophilized Standard: 2 100X Wfs1 Biotinylated Detector Anitbody: 1 x 120 uL 100X Avidin-HRP Conjugate: 1 x 120 uL Standard Diluent: 1 x 20 mL Detector Antibody Diluent: 1 x 12 mL Conjugate Diluent: 1 x 12 mL 30X Wash Buffer: 1 x 20 mL 100X Luminol Substrate: 1 x 2 mL Substrate Diluent: 1 x 20 mL
Kit detection	Chemiluminescent
Kit duration	2h, 40min
Kit principle	The microplate provided in this kit has been pre-coated with an antibody specific to Wolfram Syndrome Protein 1 (WFS1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Wolfram Syndrome Protein 1 (WFS1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Wolfram Syndrome Protein 1 (WFS1) level in the sample or standard.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wolfram Syndrome Protein 1 (WFS1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wolfram Syndrome Protein 1 (WFS1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Tissue homogenates and other biological fluids

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

OKCD04028.pdf

OKCD04028-datasheet.pdf

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Menge: 96 Wells

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Lieferung vsl. bis 30.10.2025

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