« Zurück
Startseite /
WT1 Chemi-Luminescent ELISA Kit (Human)

WT1 Chemi-Luminescent ELISA Kit (Human)

ArtNr	OKCD04048
Hersteller	AVIVA Systems Biology
Menge	96 Wells
Kategorie	Metabolism Diabetes Glucose Homeostasis ELISA ELISA & Immunoassays ELISA Kits Immunoassay CLIA
Typ	Elisa-Kit
Applikationen	CLIA, IA
Specific against	Human (Homo sapiens)
Sensitivity	0.156 ng/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	AWT1;GUD;NPHS4;WAGR;Wilms tumor 1;Wilms tumor protein;WIT-2;WT33.
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Type	ELISA Kit
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	WT1
Gene Fullname	WT1 transcription factor
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	Transcription factor that plays an important role in cellular development and cell survival (PubMed:7862533). Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3' (PubMed:7862533, PubMed:17716689, PubMed:25258363). Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors (PubMed:15520190). Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing (PubMed:16934801). Isoform 1 has lower affinity for DNA, and can bind RNA (PubMed:19123921).
Nucleotide accession_num	NM_000378
Protein accession_num	NP_000369
Protein name	Wilms tumor protein
Assay info	Assay Methodology: Quantitative Sandwich ELISA
Kit Components	Component: Amount WT1 Microplate: 96 Wells (12 x 8 Well strips) WT1 Lyophilized Standard: 2 100X WT1 Biotinylated Detector Anitbody: 1 x 120 uL 100X Avidin-HRP Conjugate: 1 x 120 uL Standard Diluent: 1 x 20 mL Detector Antibody Diluent: 1 x 12 mL Conjugate Diluent: 1 x 12 mL 30X Wash Buffer: 1 x 20 mL 100X Luminol Substrate: 1 x 2 mL Substrate Diluent: 1 x 20 mL
Kit detection	Chemiluminescent
Kit duration	2h, 40min
Kit principle	The microplate provided in this kit has been pre-coated with an antibody specific to Wilms Tumor Protein (WT1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Wilms Tumor Protein (WT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Wilms Tumor Protein (WT1) level in the sample or standard.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wilms Tumor Protein (WT1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wilms Tumor Protein (WT1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Tissue homogenates and other biological fluids

Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

OKCD04048.pdf

OKCD04048-datasheet.pdf

Sicherheitsdatenblatt anfordern

Einfache Bestellung Periodische Bestellung

Menge: 96 Wells

lieferbar

Lieferung vsl. bis 11.12.2025

Express-Lieferung: 3 Tage sparen

Vergleichen

Auf den Wunschzettel

Angebot anfordern