XPC Chemi-Luminescent ELISA Kit (Human)

ELISA Kit

Wet Ice

XPC complex subunit, DNA damage recognition and repair factor

Involved in global genome nucleotide excision repair (GG-NER) by acting as damage sensing and DNA-binding factor component of the XPC complex. Has only a low DNA repair activity by itself which is stimulated by RAD23B and RAD23A. Has a preference to bind DNA containing a short single-stranded segment but not to damaged oligonucleotides. This feature is proposed to be related to a dynamic sensor function: XPC can rapidly screen duplex DNA for non-hydrogen-bonded bases by forming a transient nucleoprotein intermediate complex which matures into a stable recognition complex through an intrinsic single-stranded DNA-binding activity.

NP_004619

Assay Methodology: Quantitative Sandwich ELISA

Chemiluminescent

The microplate provided in this kit has been pre-coated with an antibody specific to Xeroderma Pigmentosum, Complementation Group C (XPC). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Xeroderma Pigmentosum, Complementation Group C (XPC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Xeroderma Pigmentosum, Complementation Group C (XPC) level in the sample or standard.

Tissue homogenates and other biological fluids