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ZBED3 Chemi-Luminescent ELISA Kit (Human)

ArtNr OKCD04062
Hersteller AVIVA Systems Biology
Menge 96 Wells
Kategorie
Typ Elisa-Kit
Applikationen CLIA, IA
Specific against Human (Homo sapiens)
Sensitivity 0.156 ng/mL
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias 2610005H11Rik;axin-interacting protein;zinc finger BED domain-containing protein 3;zinc finger,BED domain containing 3.
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
ELISA Kit
Manufacturer - Category
Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature
Wet Ice
Gene symbol
ZBED3
Gene Fullname
zinc finger BED-type containing 3
Reconstitution and storage
2°C to 8°C|-20°C
Description of target
Acts as a positive regulator in the activation of the canonical Wnt/beta-catenin signaling pathway by stabilizing cytoplasmic beta-catenin. Involved in transcription activation of Wnt target gene expression (By similarity).
Nucleotide accession_num
NM_001329564
Protein accession_num
NP_001316493
Protein name
Zinc finger BED domain-containing protein 3
Assay info
Assay Methodology: Quantitative Sandwich ELISA
Kit Components
Component: Amount
ZBED3 Microplate: 96 Wells (12 x 8 Well strips)
ZBED3 Lyophilized Standard: 2
100X ZBED3 Biotinylated Detector Anitbody: 1 x 120 uL
100X Avidin-HRP Conjugate: 1 x 120 uL
Standard Diluent: 1 x 20 mL
Detector Antibody Diluent: 1 x 12 mL
Conjugate Diluent: 1 x 12 mL
30X Wash Buffer: 1 x 20 mL
100X Luminol Substrate: 1 x 2 mL
Substrate Diluent: 1 x 20 mL
Kit detection
Chemiluminescent
Kit duration
2h, 40min
Kit principle
The microplate provided in this kit has been pre-coated with an antibody specific to Zinc Finger, BED Type Containing Protein 3 (ZBED3). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Zinc Finger, BED Type Containing Protein 3 (ZBED3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Zinc Finger, BED Type Containing Protein 3 (ZBED3) level in the sample or standard.
Kit reproducibility
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Zinc Finger, BED Type Containing Protein 3 (ZBED3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Zinc Finger, BED Type Containing Protein 3 (ZBED3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type
Tissue homogenates, cell lysates and other biological fluids

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96 Wells
Lieferbar: In stock
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