DARC ELISA Kit (Human)

Enzyme-linked Immunosorbent assay-Sandwich

Wet Ice

atypical chemokine receptor 1 (Duffy blood group)

Atypical chemokine receptor that controls chemokine levels and localization via high-affinity chemokine binding that is uncoupled from classic ligand-driven signal transduction cascades, resulting instead in chemokine sequestration, degradation, or transcytosis. Also known as interceptor (internalizing receptor) or chemokine-scavenging receptor or chemokine decoy receptor. Has a promiscuous chemokine-binding profile, interacting with inflammatory chemokines of both the CXC and the CC subfamilies but not with homeostatic chemokines. Acts as a receptor for chemokines including CCL2, CCL5, CCL7, CCL11, CCL13, CCL14, CCL17, CXCL5, CXCL6, IL8/CXCL8, CXCL11, GRO, RANTES, MCP-1, TARC and also for the malaria parasites P.vivax and P.knowlesi. May regulate chemokine bioavailability and, consequently, leukocyte recruitment through two distinct mechanisms: when expressed in endothelial cells, it sustains the abluminal to luminal transcytosis of tissue-derived chemokines and their subsequent presentation to circulating leukocytes; when expressed in erythrocytes, serves as blood reservoir of cognate chemokines but also as a chemokine sink, buffering potential surges in plasma chemokine levels.

Assay Methodology: Quantitative Sandwich ELISA

Colorimetric

Sample - 1:2 - 1:4 - 1:8 - 1:16
Serum (n=5) - 97-105% - 80-105% - 93-102% - 96-105%
EDTA Plasma (n=5) - 81-99% - 96-104% - 81-93% - 92-104%
Heparin Plasma (n=5) - 81-93% - 79-103% - 88-95% - 94-102%

Matrix - Recovery range (%) - Average (%)
Serum (n=5) - 80-99 - 87
EDTA Plasma (n=5) - 87-97 - 92
Heparin Plasma (n=5) - 95-104 - 98

Serum, plasma, cell lysates and other biological fluids.