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CEACAM6 ELISA Kit (Human)

CEACAM6 ELISA Kit (Human)

ArtNr	OKCD04386
Hersteller	AVIVA Systems Biology
Menge	96 Wells
Kategorie	Oncology By Cell Type Carcinoma Hematology Leukemia ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Lung Cancer Bladder Cancer Colorectal Cancer Breast Cancer Testicular & Prostate Cancer Gallbladder Cancer Gastric Cancer Pancreatic Cancer Thyroid Cancer Immunostaining
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	9.68 pg/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	carcinoembryonic antigen related cell adhesion molecule 6;carcinoembryonic antigen-related cell adhesion molecule 6;carcinoembryonic antigen-related cell adhesion molecule 6 (non-specific cross reacting antigen);CD66c;CEAL;Cluster of Differentiation 66c;NCA;Non-specific crossreacting antigen;normal cross-reacting antigen.
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Type	ELISA Kit
Manufacturer - Applications	Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	CEACAM6
Gene Fullname	CEA cell adhesion molecule 6
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	Cell surface glycoprotein that plays a role in cell adhesion and tumor progression (PubMed:2803308, PubMed:2022629, PubMed:1378450, PubMed:8776764, PubMed:11590190, PubMed:10910050, PubMed:14724575, PubMed:16204051). Intercellular adhesion occurs in a calcium- and fibronectin-independent manner (PubMed:2022629, PubMed:16204051). Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM5 and CEACAM8 (PubMed:2803308, PubMed:2022629, PubMed:8776764, PubMed:11590190, PubMed:16204051). Heterophilic interaction with CEACAM8 occurs in activated neutrophils (PubMed:8776764). Plays a role in neutrophil adhesion to cytokine-activated endothelial cells (PubMed:1378450). Plays a role as an oncogene by promoting tumor progression; positively regulates cell migration, cell adhesion to endothelial cells and cell invasion (PubMed:16204051). Also involved in the metastatic cascade process by inducing gain resistance to anoikis of pancreatic adenocarcinoma and colorectal carcinoma cells (PubMed:10910050, PubMed:14724575).
Nucleotide accession_num	NM_002483.6
Protein accession_num	NP_002474.4
Protein name	Carcinoembryonic antigen-related cell adhesion molecule 6
Assay info	Assay Methodology: Quantitative Sandwich ELISA
Kit Components	Component: Amount CEACAM6 Microplate: 96 Wells (12 x 8 Well strips) CEACAM6 Lyophilized Standard: 2 100X CEACAM6 HRP-Detector Anitbody: 1 x 120 uL 100X Avidin-HRP Conjugate: 1 x 120 uL Standard Diluent: 1 x 20 mL Detector Antibody Diluent: 1 x 12 mL Conjugate Diluent: 1 x 12 mL 30X Wash Buffer: 1 x 20 mL TMB Substrate: 1 x 9 mL Stop Solution: 1 x 6 mL
Kit detection	Colorimetric
Kit duration	3h
Kit linearity	Sample - 1:2 - 1:4 - 1:8 - 1:16 Serum (n=5) - 78-90% - 78-93% - 86-105% - 78-101% EDTA Plasma (n=5) - 97-105% - 92-101% - 96-103% - 91-98% Heparin Plasma (n=5) - 93-105% - 95-103% - 87-99% - 89-102%
Kit principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carcinoembryonic Antigen Related Cell Adhesion Molecule 6 (CEACAM6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carcinoembryonic Antigen Related Cell Adhesion Molecule 6 (CEACAM6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carcinoembryonic Antigen Related Cell Adhesion Molecule 6 (CEACAM6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Carcinoembryonic Antigen Related Cell Adhesion Molecule 6 (CEACAM6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Kit recovery	Matrix - Recovery range (%) - Average (%) Serum (n=5) - 92-99 - 95 EDTA Plasma (n=5) - 81-98 - 89 Heparin Plasma (n=5) - 95-103 - 99
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Carcinoembryonic Antigen Related Cell Adhesion Molecule 6 (CEACAM6) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Carcinoembryonic Antigen Related Cell Adhesion Molecule 6 (CEACAM6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Serum, plasma and other biological fluids.

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OKCD04386.pdf

OKCD04386-datasheet.pdf

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Menge: 96 Wells

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Lieferung vsl. bis 26.02.2026

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