PRF1 ELISA Kit (Mouse)

ELISA Kit

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Prf1

2°C to 8°C|-20°C

NM_011073.3

Perforin-1

Component: Amount
CX40 Microplate: 96 Wells (12 x 8 Well strips)
CX40 Lyophilized Standard: 2
100X CX40 HRP-Detector Anitbody: 1 x 120 uL
100X Avidin-HRP Conjugate: 1 x 120 uL
Standard Diluent: 1 x 20 mL
Detector Antibody Diluent: 1 x 12 mL
Conjugate Diluent: 1 x 12 mL
30X Wash Buffer: 1 x 20 mL
100X Luminol Substrate: 1 x 2 mL
Substrate Diluent: 1 x 20 mL

3h

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Perforin 1 (PRF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Perforin 1 (PRF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Perforin 1 (PRF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Perforin 1 (PRF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids