VGLUT1 ELISA Kit (Mouse)

Enzyme-linked Immunosorbent assay-Sandwich

Wet Ice

solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7

Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate.

NP_892038.2

Assay Methodology: Quantitative Sandwich ELISA

Colorimetric

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vesicular Glutamate Transporter 1 (VGLUT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vesicular Glutamate Transporter 1 (VGLUT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vesicular Glutamate Transporter 1 (VGLUT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Vesicular Glutamate Transporter 1 (VGLUT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Tissue homogenates and other biological fluids.