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CCL11 Chemi-Luminescent ELISA Kit (Guinea Pig )

ArtNr OKCD05507
Hersteller AVIVA Systems Biology
Menge 96 Wells
Kategorie
Typ Elisa-Kit
Applikationen CLIA, IA
Specific against Guinea Pig
Sensitivity < 0.49pg/mL
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias C-C motif chemokine 11;chemokine (C-C motif) ligand 11;eosinophil chemotactic protein;eotaxin;SCYA11;small chemokine (C-C motif) ligand 11;small-inducible cytokine A11.
Versandbedingung Gekühlt
Lieferbar
Specificity Cavia porcellus, Guinea Pig
Manufacturer - Type
ELISA Kit
Manufacturer - Category
Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature
Wet Ice
Gene symbol
Ccl11
Gene Fullname
C-C motif chemokine ligand 11
Reconstitution and storage
2°C to 8°C|-20°C
Description of target
In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils, a prominent feature of allergic inflammatory reactions.
Nucleotide accession_num
NM_001172824.1
Protein accession_num
NP_001166295.1
Protein name
Eotaxin
Kit Components
Component: Amount
CCL11 Microplate: 96 Wells (12 x 8 Well strips)CCL11 Lyophilized Standard: 2100X CCL11 HRP-Detector Anitbody: 1 x 120 uL100X Avidin-HRP Conjugate: 1 x 120 uLStandard Diluent: 1 x 20 mLDetector Antibody Diluent: 1 x 12 mLConjugate Diluent: 1 x 12 mL30X Wash Buffer: 1 x 20 mL100X Luminol Substrate: 1 x 9 mLSubstrate Diluent: 1 x 6 mL
Kit detection
Chemiluminescent
Kit duration
2h, 40min
Kit principle
The microplate provided in this kit has been pre-coated with an antibody specific to Eosinophil Chemotactic Factor (ECF). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Eosinophil Chemotactic Factor (ECF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Eosinophil Chemotactic Factor (ECF) level in the sample or standard.
Kit reproducibility
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Eosinophil Chemotactic Factor (ECF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Eosinophil Chemotactic Factor (ECF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type
Serum, plasma and other biological fluids

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96 Wells
Lieferbar: In stock
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