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CGB3 MORE Chemi-Luminescent ELISA Kit (Human)

ArtNr OKCD05587
Hersteller AVIVA Systems Biology
Menge 96 Wells
Kategorie
Typ Elisa-Kit
Applikationen CLIA, IA
Specific against Human (Homo sapiens)
Sensitivity < 5.1pg/mL
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias CGB;CGB5;CGB7;CGB8;CG-beta;Choriogonadotropin subunit beta;choriogonadotropin subunit beta 3;chorionic gonadotrophin chain beta;chorionic gonadotropin beta 3 subunit;chorionic gonadotropin beta 5 subunit;chorionic gonadotropin beta 8 subunit;chorionic gonadotropin beta chain;chorionic gonadotropin beta subunit 3;chorionic gonadotropin beta subunit 5;chorionic gonadotropin beta subunit 8;chorionic gonadotropin chain beta;chorionic gonadotropin,beta polypeptide;chorionic gonadotropin,beta polypeptide 5;chorionic gonadotropin,beta polypeptide 8;HCG;hCGB;LHB;luteinizing hormone beta subunit.
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
ELISA Kit
Manufacturer - Category
Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature
Wet Ice
Gene symbol
CGB3|CGB5|CGB8
Gene Fullname
chorionic gonadotropin subunit beta 3|chorionic gonadotropin subunit beta 5|chorionic gonadotropin subunit beta 8
Reconstitution and storage
2°C to 8°C|-20°C
Description of target
Beta subunit of the human chorionic gonadotropin (hCG). hCG is a complex glycoprotein composed of two glycosylated subunits alpha and beta which are non-covalently associated. The alpha subunit is identical to those in the pituitary gonadotropin hormones (LH, FSH and TSH). The beta subunits are distinct in each of the hormones and confer receptor and biological specificity. Has an essential role in pregnancy and maternal adaptation. Stimulates the ovaries to synthesize the steroids that are essential for the maintenance of pregnancy.
Nucleotide accession_num
NM_000737.3
Protein accession_num
NP_000728.1
Protein name
Choriogonadotropin subunit beta 3
Kit Components
Component: Amount
CGB3 MORE Microplate: 96 Wells (12 x 8 Well strips)CGB3 MORE Lyophilized Standard: 2100X CGB3 MORE HRP-Detector Anitbody: 1 x 120 uL100X Avidin-HRP Conjugate: 1 x 120 uLStandard Diluent: 1 x 20 mLDetector Antibody Diluent: 1 x 12 mLConjugate Diluent: 1 x 12 mL30X Wash Buffer: 1 x 20 mL100X Luminol Substrate: 1 x 9 mLSubstrate Diluent: 1 x 6 mL
Kit detection
Chemiluminescent
Kit duration
2h, 40min
Kit principle
The microplate provided in this kit has been pre-coated with an antibody specific to Chorionic Gonadotropin (CG). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Chorionic Gonadotropin (CG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Chorionic Gonadotropin (CG) level in the sample or standard.
Kit reproducibility
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chorionic Gonadotropin (CG) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chorionic Gonadotropin (CG) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type
Serum, plasma, urine, cell culture supernates and other biological fluids

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96 Wells
Lieferbar: In stock
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