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KL Chemi-Luminescent ELISA Kit (Human)

KL Chemi-Luminescent ELISA Kit (Human)

ArtNr	OKCD05590
Hersteller	AVIVA Systems Biology
Menge	96 Wells
Kategorie	Oncology By Cell Type Carcinoma Hematology Myeloma ELISA ELISA & Immunoassays ELISA Kits Immunoassay CLIA
Typ	Elisa-Kit
Applikationen	CLIA, IA
Specific against	Human (Homo sapiens)
Sensitivity	< 16.8pg/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	alpha-klotho;HFTC3;KLA;klotho.
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Type	ELISA Kit
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	KL
Gene Fullname	klotho
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D (By similarity). Essential factor for the specific interaction between FGF23 and FGFR1 (By similarity).
Nucleotide accession_num	NM_004795.3
Protein accession_num	NP_004786.2
Protein name	Klotho
Kit Components	Component: Amount KL Microplate: 96 Wells (12 x 8 Well strips)KL Lyophilized Standard: 2100X KL HRP-Detector Anitbody: 1 x 120 uL100X Avidin-HRP Conjugate: 1 x 120 uLStandard Diluent: 1 x 20 mLDetector Antibody Diluent: 1 x 12 mLConjugate Diluent: 1 x 12 mL30X Wash Buffer: 1 x 20 mL100X Luminol Substrate: 1 x 2 mLSubstrate Diluent: 1 x 20 mL
Kit detection	Chemiluminescent
Kit duration	2h, 40min
Kit principle	The microplate provided in this kit has been pre-coated with an antibody specific to Klotho (KL). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Klotho (KL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Klotho (KL) level in the sample or standard.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Klotho (KL) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Klotho (KL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Serum, plasma, tissue homogenates and other biological fluids

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

OKCD05590.pdf

OKCD05590-datasheet.pdf

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Menge: 96 Wells

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Lieferung vsl. bis 11.12.2025

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