FAS ELISA Kit (Rat)

ArtNr	OKCD05672
Hersteller	AVIVA Systems Biology
Menge	96 Wells
Kategorie	Oncology Metabolism Cholesterol & Lipid Metabolism By Cell Type Carcinoma Cell Biology Cell Status Apoptosis ELISA Enzymes Fatty Acid Synthesis ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Rat (Rattus norvegicus)
Sensitivity	< 6.7pg/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	apo-1 antigen;apoptosis-mediating surface antigen FAS;CD95 antigen;Fas (TNF receptor superfamily,member 6);Fas antigen (ATP1);Fas receptor;FASLG receptor;Tnfrsf6;tumor necrosis factor receptor superfamily member 6;Tumor necrosis factor receptor superfamily,member 6.
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Type	ELISA Kit
Manufacturer - Applications	Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	Fas
Gene Fullname	Fas cell surface death receptor
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both (By similarity).
Nucleotide accession_num	NM_139194.2
Protein accession_num	NP_631933.2
Protein name	Tumor necrosis factor receptor superfamily member 6
Kit Components	Component: Amount FAS Microplate: 96 Wells (12 x 8 Well strips)FAS Lyophilized Standard: 2100X FAS HRP-Detector Anitbody: 1 x 120 uL100X Avidin-HRP Conjugate: 1 x 120 uLStandard Diluent: 1 x 20 mLDetector Antibody Diluent: 1 x 12 mLConjugate Diluent: 1 x 12 mL30X Wash Buffer: 1 x 20 mLTMB Substrate: 1 x 2 mLStop Solution: 1 x 20 mL
Kit detection	Colorimetric
Kit duration	3h
Kit principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Factor Related Apoptosis (FAS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Factor Related Apoptosis (FAS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Factor Related Apoptosis (FAS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Factor Related Apoptosis (FAS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Factor Related Apoptosis (FAS) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Factor Related Apoptosis (FAS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Serum, plasma, tissue homogenates, cell lysates and other biological fluids.

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OKCD05672.pdf

OKCD05672-datasheet.pdf

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Menge: 96 Wells

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