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FASLG ELISA Kit (Human)

FASLG ELISA Kit (Human)

ArtNr	OKCD05673
Hersteller	AVIVA Systems Biology
Menge	96 Wells
Kategorie	Cell Biology Cell Status Apoptosis ELISA ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	< 6.2pg/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	ALPS1B;apoptosis (APO-1) antigen ligand 1;apoptosis antigen ligand;APT1LG1;APTL;CD178;CD95 ligand;CD95L;CD95-L;fas antigen ligand;Fas ligand (TNF superfamily,member 6);FASL;mutant tumor necrosis factor family member 6;TNFSF6;TNLG1A;tumor necrosis factor ligand 1A;tumor necrosis factor ligand superfamily member 6.
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Type	ELISA Kit
Manufacturer - Applications	Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	FASLG
Gene Fullname	Fas ligand
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells (PubMed:26334989, PubMed:9228058). Involved in cytotoxic T-cell-mediated apoptosis, natural killer cell-mediated apoptosis and in T-cell development (PubMed:9228058, PubMed:7528780, PubMed:9427603). Initiates fratricidal/suicidal activation-induced cell death (AICD) in antigen-activated T-cells contributing to the termination of immune responses (By similarity). TNFRSF6/FAS-mediated apoptosis has also a role in the induction of peripheral tolerance (By similarity). Binds to TNFRSF6B/DcR3, a decoy receptor that blocks apoptosis (PubMed:27806260).
Nucleotide accession_num	NM_000639.2
Protein accession_num	NP_000630.1
Protein name	Tumor necrosis factor ligand superfamily member 6
Kit Components	Component: Amount FASLG Microplate: 96 Wells (12 x 8 Well strips)FASLG Lyophilized Standard: 2100X FASLG HRP-Detector Anitbody: 1 x 120 uL100X Avidin-HRP Conjugate: 1 x 120 uLStandard Diluent: 1 x 20 mLDetector Antibody Diluent: 1 x 12 mLConjugate Diluent: 1 x 12 mL30X Wash Buffer: 1 x 20 mLTMB Substrate: 1 x 9 mLStop Solution: 1 x 6 mL
Kit detection	Colorimetric
Kit duration	3h
Kit principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Factor Related Apoptosis Ligand (FASL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Factor Related Apoptosis Ligand (FASL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Factor Related Apoptosis Ligand (FASL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Factor Related Apoptosis Ligand (FASL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Factor Related Apoptosis Ligand (FASL) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Factor Related Apoptosis Ligand (FASL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

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OKCD05673.pdf

OKCD05673-datasheet.pdf

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Menge: 96 Wells

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