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ADIPOQ ELISA Kit (Bovine)

ADIPOQ ELISA Kit (Bovine)

ArtNr	OKCD06575
Hersteller	AVIVA Systems Biology
Menge	96 Wells
Kategorie	Oncology Neuroscience Neuroinflammation Metabolism Diabetes By Cell Type Cell Biology Obesity Cell Culture Cytokines Cell Status Necrosis Apoptosis ELISA ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Cow (Bos taurus), Cattle (Bovine)
Sensitivity	< 0.33ng/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	30 kDa adipocyte complement-related protein;ACRP30;adipocyte complement related 30kDa protein;adipocyte complement related protein of 30 kDa;adipocyte complement-related 30 kDa protein;adipocyte,C1q and collagen domain-containing protein;adiponectin;adipose most abundant gene transcript 1 protein;apM-1;APM1.
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Type	ELISA Kit
Manufacturer - Applications	Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	ADIPOQ
Gene Fullname	adiponectin, C1Q and collagen domain containing
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW (By similarity).
Nucleotide accession_num	NM_174742.2
Protein accession_num	NP_777167.1
Protein name	Adiponectin
Kit Components	Component: Amount ADIPOQ Microplate: 96 Wells (12 x 8 Well strips)ADIPOQ Lyophilized Standard: 2100X ADIPOQ HRP-Detector Anitbody: 1 x 120 uL100X Avidin-HRP Conjugate: 1 x 120 uLStandard Diluent: 1 x 20 mLDetector Antibody Diluent: 1 x 12 mLConjugate Diluent: 1 x 12 mL30X Wash Buffer: 1 x 20 mLTMB Substrate: 1 x 9 mLStop Solution: 1 x 6 mL
Kit detection	Colorimetric
Kit duration	3h
Kit principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adiponectin (ADPN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adiponectin (ADPN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adiponectin (ADPN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Adiponectin (ADPN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adiponectin (ADPN) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adiponectin (ADPN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Serum, plasma and other biological fluids

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OKCD06575.pdf

OKCD06575-datasheet.pdf

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Menge: 96 Wells

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Lieferung vsl. bis 30.10.2025

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