CP ELISA Kit (Mouse)

ArtNr	OKCD06982
Hersteller	AVIVA Systems Biology
Menge	96 Wells
Kategorie	Neuroscience Neural Signaling ELISA ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Mouse (Murine, Mus musculus)
Sensitivity	< 0.27ng/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	ceruloplasmin;D3Ertd555;D3Ertd555e;ferroxidase.
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Type	ELISA Kit
Manufacturer - Applications	Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	Cp
Gene Fullname	ceruloplasmin
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	Ceruloplasmin is a blue, copper-binding (6-7 atoms per molecule) glycoprotein. It has ferroxidase activity oxidizing Fe(2+) to Fe(3+) without releasing radical oxygen species. It is involved in iron transport across the cell membrane. Provides Cu(2+) ions for the ascorbate-mediated deaminase degradation of the heparan sulfate chains of GPC1. May also play a role in fetal lung development or pulmonary antioxidant defense (By similarity).
Nucleotide accession_num	NM_001276248.1
Protein accession_num	NP_001263177.1
Protein name	Ceruloplasmin
Kit Components	Component: Amount CP Microplate: 96 Wells (12 x 8 Well strips)CP Lyophilized Standard: 2100X CP HRP-Detector Anitbody: 1 x 120 Ã‚uL100X Avidin-HRP Conjugate: 1 x 120 Ã‚uLStandard Diluent: 1 x 20 mLDetector Antibody Diluent: 1 x 12 mLConjugate Diluent: 1 x 12 mL30X Wash Buffer: 1 x 20 mLTMB Substrate: 1 x 2 mLStop Solution: 1 x 20 mL
Kit detection	Colorimetric
Kit duration	3h
Kit principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ceruloplasmin (CP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ceruloplasmin (CP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ceruloplasmin (CP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Ceruloplasmin (CP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ceruloplasmin (CP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ceruloplasmin (CP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Serum, plasma and other biological fluids.

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OKCD06982.pdf

OKCD06982-datasheet.pdf

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Menge: 96 Wells

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