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GHRL ELISA Kit (Mouse)

GHRL ELISA Kit (Mouse)

ArtNr	OKCD07042
Hersteller	AVIVA Systems Biology
Menge	96 Wells
Kategorie	Metabolism Diabetes Glucose Homeostasis Regulation of Appetite Obesity ELISA ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Mouse (Murine, Mus musculus)
Sensitivity	< 32pg/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	2210006E23Rik;appetite-regulating hormone;Gh;Ghr;growth hormone secretagogue;growth hormone-releasing peptide;m46;motilin-related peptide;MT;MTLRP;MTLRPAP;preproghrelin;proghrelin;Protein M46.
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Type	ELISA Kit
Manufacturer - Applications	Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	Ghrl
Gene Fullname	ghrelin
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR). Induces the release of growth hormone from the pituitary. Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation.
Nucleotide accession_num	NM_001286404.1
Protein accession_num	NP_001273333.1
Protein name	Appetite-regulating hormone
Kit Components	Component: Amount GHRL Microplate: 96 Wells (12 x 8 Well strips)GHRL Lyophilized Standard: 2100X GHRL HRP-Detector Anitbody: 1 x 120 uL100X Avidin-HRP Conjugate: 1 x 120 uLStandard Diluent: 1 x 20 mLDetector Antibody Diluent: 1 x 12 mLConjugate Diluent: 1 x 12 mL30X Wash Buffer: 1 x 20 mLTMB Substrate: 1 x 2 mLStop Solution: 1 x 20 mL
Kit detection	Colorimetric
Kit duration	3h
Kit principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ghrelin (GHRL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ghrelin (GHRL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ghrelin (GHRL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Ghrelin (GHRL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ghrelin (GHRL) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ghrelin (GHRL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

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OKCD07042.pdf

OKCD07042-datasheet.pdf

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Menge: 96 Wells

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Lieferung vsl. bis 30.10.2025

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