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CD59A ELISA Kit (Mouse)

CD59A ELISA Kit (Mouse)

ArtNr	OKCD07332
Hersteller	AVIVA Systems Biology
Menge	96 Wells
Kategorie	Neuroscience Neuroinflammation ELISA ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Mouse (Murine, Mus musculus)
Sensitivity	< 25.5pg/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	AA987121;Cd59;CD59 antigen;CD59A glycoprotein;MACIF;MAC-inhibitory protein;MAC-IP;membrane attack complex inhibition factor;prot;protectin.
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Type	ELISA Kit
Manufacturer - Applications	Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	Cd59a
Gene Fullname	CD59a antigen
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore (By similarity).
Nucleotide accession_num	NM_001111060.2
Protein accession_num	NP_001104530.1
Protein name	CD59A glycoprotein
Kit Components	Component: Amount CD59A Microplate: 96 Wells (12 x 8 Well strips)CD59A Lyophilized Standard: 2100X CD59A HRP-Detector Anitbody: 1 x 120 uL100X Avidin-HRP Conjugate: 1 x 120 uLStandard Diluent: 1 x 20 mLDetector Antibody Diluent: 1 x 12 mLConjugate Diluent: 1 x 12 mL30X Wash Buffer: 1 x 20 mLTMB Substrate: 1 x 2 mLStop Solution: 1 x 20 mL
Kit detection	Colorimetric
Kit duration	3h
Kit principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cluster of Differentiation 59 (CD59). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cluster of Differentiation 59 (CD59). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cluster of Differentiation 59 (CD59), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cluster of Differentiation 59 (CD59) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cluster of Differentiation 59 (CD59) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cluster of Differentiation 59 (CD59) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Serum, plasma, urine and other biological fluids.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

OKCD07332.pdf

OKCD07332-datasheet.pdf

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Menge: 96 Wells

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Lieferung vsl. bis 25.12.2025

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