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CD63 ELISA Kit (Human)

CD63 ELISA Kit (Human)

ArtNr	OKCD07341
Hersteller	AVIVA Systems Biology
Menge	96 Wells
Kategorie	Oncology Neuroscience Neural Signaling Neural Adhesion Molecules By Cell Type Blastoma Carcinoma Hematology Lymphoma Myeloma Melanoma Sarcoma Cell Biology Cell Movement Cell Adhesion Cell Culture Cytokines Adenocarcinoma ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Bladder Cancer Immunostaining
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	< 0.055ng/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	CD63 antigen;CD63 antigen (melanoma 1 antigen);granulophysin;LAMP-3;lysosomal-associated membrane protein 3;lysosome-associated membrane glycoprotein 3;ME491;melanoma-associated antigen ME491;melanoma-associated antigen MLA1;MLA1;ocular melanoma-associated antigen;OMA81H;tetraspanin-30;tspan-30;TSPAN30.
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Type	ELISA Kit
Manufacturer - Applications	Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	CD63
Gene Fullname	CD63 molecule
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	Functions as cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli.
Nucleotide accession_num	NM_001257389.1
Protein accession_num	NP_001244318.1
Protein name	CD63 antigen
Kit Components	Component: Amount CD63 Microplate: 96 Wells (12 x 8 Well strips)CD63 Lyophilized Standard: 2100X CD63 HRP-Detector Anitbody: 1 x 120 uL100X Avidin-HRP Conjugate: 1 x 120 uLStandard Diluent: 1 x 20 mLDetector Antibody Diluent: 1 x 12 mLConjugate Diluent: 1 x 12 mL30X Wash Buffer: 1 x 20 mLTMB Substrate: 1 x 9 mLStop Solution: 1 x 6 mL
Kit detection	Colorimetric
Kit duration	3h
Kit principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tetraspanin 30Cluster of Differentiation 63 (CD63). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tetraspanin 30Cluster of Differentiation 63 (CD63). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tetraspanin 30Cluster of Differentiation 63 (CD63), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Tetraspanin 30Cluster of Differentiation 63 (CD63) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tetraspanin 30Cluster of Differentiation 63 (CD63) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tetraspanin 30Cluster of Differentiation 63 (CD63) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Plasma, tissue homogenates, cell lysates and other biological fluids

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OKCD07341.pdf

OKCD07341-datasheet.pdf

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Menge: 96 Wells

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