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NAIP ELISA Kit (Human)

NAIP ELISA Kit (Human)

ArtNr	OKCD07467
Hersteller	AVIVA Systems Biology
Menge	96 Wells
Kategorie	Oncology Neuroscience Molecular Targets for Neuroscience Aging & Neurological Disorders Neural Epigenetics By Cell Type Carcinoma Cell Biology Cell Status Apoptosis ELISA ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	< 0.058ng/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	baculoviral IAP repeat-containing protein 1;BIRC1;neuronal apoptosis inhibitory protein;NLRB1;nucleotide-binding oligomerization domain,leucine rich repeat and BIR domain containing 1;psi neuronal apoptosis inhibitory protein;psiNAIP.
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Type	ELISA Kit
Manufacturer - Applications	Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	NAIP
Gene Fullname	NLR family apoptosis inhibitory protein
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	Anti-apoptotic protein which acts by inhibiting the activities of CASP3, CASP7 and CASP9. Can inhibit the autocleavage of pro-CASP9 and cleavage of pro-CASP3 by CASP9. Capable of inhibiting CASP9 autoproteolysis at 'Asp-315' and decreasing the rate of auto proteolysis at 'Asp-330'. Acts as a mediator of neuronal survival in pathological conditions. Prevents motor-neuron apoptosis induced by a variety of signals. Possible role in the prevention of spinal muscular atrophy that seems to be caused by inappropriate persistence of motor-neuron apoptosis: mutated or deleted forms of NAIP have been found in individuals with severe spinal muscular atrophy.
Nucleotide accession_num	NM_001346870.1
Protein accession_num	NP_001333799.1
Protein name	Baculoviral IAP repeat-containing protein 1
Kit Components	Component: Amount NAIP Microplate: 96 Wells (12 x 8 Well strips)NAIP Lyophilized Standard: 2100X NAIP HRP-Detector Anitbody: 1 x 120 uL100X Avidin-HRP Conjugate: 1 x 120 uLStandard Diluent: 1 x 20 mLDetector Antibody Diluent: 1 x 12 mLConjugate Diluent: 1 x 12 mL30X Wash Buffer: 1 x 20 mLTMB Substrate: 1 x 9 mLStop Solution: 1 x 6 mL
Kit detection	Colorimetric
Kit duration	3h
Kit principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neuronal Apoptosis Inhibitory Protein (NAIP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neuronal Apoptosis Inhibitory Protein (NAIP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neuronal Apoptosis Inhibitory Protein (NAIP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Neuronal Apoptosis Inhibitory Protein (NAIP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neuronal Apoptosis Inhibitory Protein (NAIP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neuronal Apoptosis Inhibitory Protein (NAIP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Tissue homogenates and other biological fluids.

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OKCD07467.pdf

OKCD07467-datasheet.pdf

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Menge: 96 Wells

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