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NRG2 ELISA Kit (Rat)

ArtNr OKCD08033
Hersteller AVIVA Systems Biology
Menge 96 Wells
Kategorie
Typ Elisa-Kit
Applikationen ELISA
Specific against Rat (Rattus norvegicus)
Sensitivity < 0.062ng/mL
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias neural- and thymus-derived activator for ErbB kinases;Ntak;NTAK alpha1;NTAK alpha2a;NTAK_alpha2a;pro-neuregulin-2,membrane-bound isoform;pro-NRG2.
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
ELISA Kit
Manufacturer - Applications
Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category
Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature
Wet Ice
Gene symbol
Nrg2
Gene Fullname
neuregulin 2
Reconstitution and storage
2°C to 8°C|-20°C
Description of target
Direct ligand for ERBB3 and ERBB4 tyrosine kinase receptors. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. May also promote the heterodimerization with the EGF receptor.
Nucleotide accession_num
NM_001136151.1
Protein accession_num
NP_001129623.1
Protein name
Pro-neuregulin-2, membrane-bound isoform
Kit Components
Component: Amount
NRG2 Microplate: 96 Wells (12 x 8 Well strips)NRG2 Lyophilized Standard: 2100X NRG2 HRP-Detector Anitbody: 1 x 120 uL100X Avidin-HRP Conjugate: 1 x 120 uLStandard Diluent: 1 x 20 mLDetector Antibody Diluent: 1 x 12 mLConjugate Diluent: 1 x 12 mL30X Wash Buffer: 1 x 20 mLTMB Substrate: 1 x 9 mLStop Solution: 1 x 6 mL
Kit detection
Colorimetric
Kit duration
3h
Kit principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neuregulin 2 (NRG2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neuregulin 2 (NRG2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neuregulin 2 (NRG2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Neuregulin 2 (NRG2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Kit reproducibility
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neuregulin 2 (NRG2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neuregulin 2 (NRG2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type
Serum, plasma, tissue homogenates and other biological fluids.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96 Wells
Lieferbar: In stock
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Lieferung vsl. bis 30.10.2025 

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