MID1 ELISA Kit (Mouse)

ELISA Kit

Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit

Mid1

2°C to 8°C|-20°C

NM_001290504.1

E3 ubiquitin-protein ligase Midline-1

Colorimetric

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Midline 1 (MID1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Midline 1 (MID1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Midline 1 (MID1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Midline 1 (MID1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Tissue homogenates and other biological fluids.