SNX9 ELISA Kit (Human)

ArtNr	OKCD08725
Hersteller	AVIVA Systems Biology
Menge	96 Wells
Kategorie	Oncology Neuroscience Neural Signaling By Cell Type Blastoma Carcinoma Glioma Hematology Lymphoma Myeloma Melanoma Sarcoma Cell Biology Cell Culture Cytokines Adenocarcinoma ELISA ELISA & Immunoassays ELISA Kits Immunoassay Astrocytoma By Organ Bladder Cancer Immunostaining
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	< 0.052ng/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	SDP1;SH3 and PX domain-containing protein 1;SH3 and PX domain-containing protein 3A;SH3PX1;SH3PXD3A;sorting nexin-9;Wiskott-Aldrich syndrome protein (WASP) interactor protein;WISP.
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Type	ELISA Kit
Manufacturer - Applications	Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	SNX9
Gene Fullname	sorting nexin 9
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	Involved in endocytosis and intracellular vesicle trafficking, both during interphase and at the end of mitosis. Required for efficient progress through mitosis and cytokinesis. Required for normal formation of the cleavage furrow at the end of mitosis. Plays a role in endocytosis via clathrin-coated pits, but also clathrin-independent, actin-dependent fluid-phase endocytosis. Plays a role in macropinocytosis. Promotes internalization of TNFR. Promotes degradation of EGFR after EGF signaling. Stimulates the GTPase activity of DNM1. Promotes DNM1 oligomerization. Promotes activation of the Arp2/3 complex by WASL, and thereby plays a role in the reorganization of the F-actin cytoskeleton. Binds to membranes enriched in phosphatidylinositol 4, 5-bisphosphate and promotes membrane tubulation. Has lower affinity for membranes enriched in phosphatidylinositol 3-phosphate.
Nucleotide accession_num	NM_016224.4
Protein accession_num	NP_057308.1
Protein name	Sorting nexin-9
Kit Components	Component: Amount SNX9 Microplate: 96 Wells (12 x 8 Well strips)SNX9 Lyophilized Standard: 2100X SNX9 HRP-Detector Anitbody: 1 x 120 uL100X Avidin-HRP Conjugate: 1 x 120 uLStandard Diluent: 1 x 20 mLDetector Antibody Diluent: 1 x 12 mLConjugate Diluent: 1 x 12 mL30X Wash Buffer: 1 x 20 mLTMB Substrate: 1 x 9 mLStop Solution: 1 x 6 mL
Kit detection	Colorimetric
Kit duration	3h
Kit principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sorting Nexin 9 (SNX9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sorting Nexin 9 (SNX9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sorting Nexin 9 (SNX9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Sorting Nexin 9 (SNX9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sorting Nexin 9 (SNX9) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sorting Nexin 9 (SNX9) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Serum, plasma, tissue homogenates and other biological fluids.

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OKCD08725.pdf

OKCD08725-datasheet.pdf

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Menge: 96 Wells

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Lieferung vsl. bis 30.10.2025