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CAPN6 ELISA Kit (Human)

CAPN6 ELISA Kit (Human)

ArtNr	OKCD08759
Hersteller	AVIVA Systems Biology
Menge	96 Wells
Kategorie	Cell Biology Cytoskeleton ELISA ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining small GTPases Rho Subfamily
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	< 0.117ng/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	calpain-6;calpain-like protease X-linked;calpamodulin;CalpM;CANPX;CAPNX;DJ914P14.1.
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Type	ELISA Kit
Manufacturer - Applications	Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	CAPN6
Gene Fullname	calpain 6
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	Microtubule-stabilizing protein that may be involved in the regulation of microtubule dynamics and cytoskeletal organization. May act as a regulator of RAC1 activity through interaction with ARHGEF2 to control lamellipodial formation and cell mobility. Does not seem to have protease activity as it has lost the active site residues (By similarity).
Nucleotide accession_num	NM_014289.3
Protein accession_num	NP_055104.2
Protein name	Calpain-6
Kit Components	Component: Amount CAPN6 Microplate: 96 Wells (12 x 8 Well strips)CAPN6 Lyophilized Standard: 2100X CAPN6 HRP-Detector Anitbody: 1 x 120 uL100X Avidin-HRP Conjugate: 1 x 120 uLStandard Diluent: 1 x 20 mLDetector Antibody Diluent: 1 x 12 mLConjugate Diluent: 1 x 12 mL30X Wash Buffer: 1 x 20 mLTMB Substrate: 1 x 9 mLStop Solution: 1 x 6 mL
Kit detection	Colorimetric
Kit duration	3h
Kit principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calpain 6 (CAPN6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calpain 6 (CAPN6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calpain 6 (CAPN6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Calpain 6 (CAPN6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calpain 6 (CAPN6) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calpain 6 (CAPN6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Tissue homogenates and other biological fluids.

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OKCD08759.pdf

OKCD08759-datasheet.pdf

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Menge: 96 Wells

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Lieferung vsl. bis 25.12.2025

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