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ATP6AP2 ELISA Kit (Human)

ATP6AP2 ELISA Kit (Human)

ArtNr	OKCD08927
Hersteller	AVIVA Systems Biology
Menge	96 Wells
Kategorie	ELISA Cardiovascular Research Heart Failure Myocardial Infarction Hypertension ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	< 0.115ng/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	APT6M8-9;ATP6IP2;ATP6M8-9;ATPase H(+)-transporting lysosomal accessory protein 2;ATPase H(+)-transporting lysosomal-interacting protein 2;ATPase,H+ transporting,lysosomal (vacuolar proton pump) membrane sector associated protein M8-9;ATPase,H+ transporting,lysosomal accessory protein 2;ATPase,H+ transporting,lysosomal interacting protein 2;CDG2R;ELDF10;embryonic liver differentiation factor 10;ER-localized type I transmembrane adapter;ER-localized type I transmembrane adaptor;HT028;M8-9;MRXE;MRXSH;MSTP009;N14F;prorenin receptor;PRR;renin receptor;renin/prorenin receptor;RENR;vacuolar ATP synthase membrane sector-associated protein M8-9;vacuolar proton ATP synthase membrane sector associated protein M8-9;V-ATPase M8.9 subunit;XMRE;XPDS.
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Type	ELISA Kit
Manufacturer - Applications	Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	ATP6AP2
Gene Fullname	ATPase H+ transporting accessory protein 2
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	Functions as a renin and prorenin cellular receptor. May mediate renin-dependent cellular responses by activating ERK1 and ERK2. By increasing the catalytic efficiency of renin in AGT/angiotensinogen conversion to angiotensin I, it may also play a role in the renin-angiotensin system (RAS).
Nucleotide accession_num	NM_005765.2
Protein accession_num	NP_005756.2
Protein name	Renin receptor
Kit Components	Component: Amount ATP6AP2 Microplate: 96 Wells (12 x 8 Well strips)ATP6AP2 Lyophilized Standard: 2100X ATP6AP2 HRP-Detector Anitbody: 1 x 120 uL100X Avidin-HRP Conjugate: 1 x 120 uLStandard Diluent: 1 x 20 mLDetector Antibody Diluent: 1 x 12 mLConjugate Diluent: 1 x 12 mL30X Wash Buffer: 1 x 20 mLTMB Substrate: 1 x 9 mLStop Solution: 1 x 6 mL
Kit detection	Colorimetric
Kit duration	3h
Kit principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Tissue homogenates, cell lysates and other biological fluids

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OKCD08927.pdf

OKCD08927-datasheet.pdf

OKCD08927-safety-datasheet.pdf