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SEMG1 ELISA Kit (Human)

SEMG1 ELISA Kit (Human)

ArtNr	OKCD09037
Hersteller	AVIVA Systems Biology
Menge	96 Wells
Kategorie	Oncology Neuroscience Molecular Targets for Neuroscience ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Testicular & Prostate Cancer Immunostaining
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	< 6.2pg/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	cancer/testis antigen 103;CT103;dJ172H20.2;semen coagulating protein;semenogelin I;semenogelin-1;SEMG;SGI;SgI-29.
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Type	ELISA Kit
Manufacturer - Applications	Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	SEMG1
Gene Fullname	semenogelin 1
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	Predominant protein in semen. It participates in the formation of a gel matrix entrapping the accessory gland secretions and ejaculated spermatozoa. Fragments of semenogelin and/or fragments of the related proteins may contribute to the activation of progressive sperm movements as the gel-forming proteins are fragmented by KLK3/PSA.
Nucleotide accession_num	NM_003007.4
Protein accession_num	NP_002998.1
Protein name	Semenogelin-1
Kit Components	Component: Amount SEMG1 Microplate: 96 Wells (12 x 8 Well strips)SEMG1 Lyophilized Standard: 2100X SEMG1 HRP-Detector Anitbody: 1 x 120 uL100X Avidin-HRP Conjugate: 1 x 120 uLStandard Diluent: 1 x 20 mLDetector Antibody Diluent: 1 x 12 mLConjugate Diluent: 1 x 12 mL30X Wash Buffer: 1 x 20 mLTMB Substrate: 1 x 9 mLStop Solution: 1 x 6 mL
Kit detection	Colorimetric
Kit duration	3h
Kit principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Semenogelin I (SEMG1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Semenogelin I (SEMG1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Semenogelin I (SEMG1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Semenogelin I (SEMG1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Semenogelin I (SEMG1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Semenogelin I (SEMG1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Serum, plasma, seminal plasma and other biological fluids

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OKCD09037.pdf

OKCD09037-datasheet.pdf

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Menge: 96 Wells

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Lieferung vsl. bis 25.12.2025

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