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KLB ELISA Kit (Human)

KLB ELISA Kit (Human)

ArtNr	OKCD09093
Hersteller	AVIVA Systems Biology
Menge	96 Wells
Kategorie	Oncology Neuroscience Neural Lineage Markers Metabolism Diabetes Glucose Homeostasis Neural Signaling Neural Adhesion Molecules By Cell Type Blastoma Carcinoma Glioma Hematology Leukemia Lymphoma Myeloma Sarcoma Cell Biology Obesity Cell Culture Cytokines ELISA ELISA & Immunoassays ELISA Kits Immunoassay Astrocytoma Immunostaining Calcium Binding Protein Markers Interneuron Markers
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	< 29pg/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	beta-klotho;betaKlotho;BKL;b-Klotho;klotho beta like;klotho beta-like protein.
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Type	ELISA Kit
Manufacturer - Applications	Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	KLB
Gene Fullname	klotho beta
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	Contributes to the transcriptional repression of cholesterol 7-alpha-hydroxylase (CYP7A1), the rate-limiting enzyme in bile acid synthesis. Probably inactive as a glycosidase. Increases the ability of FGFR1 and FGFR4 to bind FGF21 (By similarity).
Nucleotide accession_num	NM_175737.3
Protein accession_num	NP_783864.1
Protein name	Beta-klotho
Kit Components	Component: Amount KLB Microplate: 96 Wells (12 x 8 Well strips)KLB Lyophilized Standard: 2100X KLB HRP-Detector Anitbody: 1 x 120 uL100X Avidin-HRP Conjugate: 1 x 120 uLStandard Diluent: 1 x 20 mLDetector Antibody Diluent: 1 x 12 mLConjugate Diluent: 1 x 12 mL30X Wash Buffer: 1 x 20 mLTMB Substrate: 1 x 9 mLStop Solution: 1 x 6 mL
Kit detection	Colorimetric
Kit duration	3h
Kit principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Klotho Beta (KLb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Klotho Beta (KLb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Klotho Beta (KLb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Klotho Beta (KLb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Klotho Beta (KLb) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Klotho Beta (KLb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Serum, plasma and other biological fluids

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OKCD09093.pdf

OKCD09093-datasheet.pdf

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Einfache Bestellung Periodische Bestellung

Menge: 96 Wells

lieferbar

Lieferung vsl. bis 30.10.2025

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