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INPP5D ELISA Kit (Human)

INPP5D ELISA Kit (Human)

ArtNr	OKCD09350
Hersteller	AVIVA Systems Biology
Menge	96 Wells
Kategorie	Oncology Neuroscience Neural Signaling By Cell Type Blastoma Carcinoma Glioma Hematology Leukemia Lymphoma Myeloma Melanoma Sarcoma Cell Biology Cell Culture Cytokines Cell Status Adenocarcinoma Mesothelioma ELISA Autophagy Cardiovascular Research Proliferation Myocardial Infarction ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	< 13.5pg/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	hp51CN;Inositol polyphosphate-5-phosphatase of 145 kDa;inositol polyphosphate-5-phosphatase,145kD;inositol polyphosphate-5-phosphatase,145kDa;p150Ship;phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1;phosphatidylinositol 4,5-bisphosphate 5-phosphatase;SH2 domain-containing inositol 5'-phosphatase 1;SHIP;SHIP1;SHIP-1;signaling inositol polyphosphate 5 phosphatase SIP-145;signaling inositol polyphosphate phosphatase SHIP II;SIP-145.
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Type	ELISA Kit
Manufacturer - Applications	Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	INPP5D
Gene Fullname	inositol polyphosphate-5-phosphatase D
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	Phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3, 4, 5-trisphosphate (PtdIns(3, 4, 5)P3) to produce PtdIns(3, 4)P2, thereby negatively regulating the PI3K (phosphoinositide 3-kinase) pathways. Acts as a negative regulator of B-cell antigen receptor signaling. Mediates signaling from the FC-gamma-RIIB receptor (FCGR2B), playing a central role in terminating signal transduction from activating immune/hematopoietic cell receptor systems. Acts as a negative regulator of myeloid cell proliferation/survival and chemotaxis, mast cell degranulation, immune cells homeostasis, integrin alpha-IIb/beta-3 signaling in platelets and JNK signaling in B-cells. Regulates proliferation of osteoclast precursors, macrophage programming, phagocytosis and activation and is required for endotoxin tolerance. Involved in the control of cell-cell junctions, CD32a signaling in neutrophils and modulation of EGF-induced phospholipase C activity. Key regulator of neutrophil migration, by governing the formation of the leading edge and polarization required for chemotaxis. Modulates FCGR3/CD16-mediated cytotoxicity in NK cells. Mediates the activin/TGF-beta-induced apoptosis through its Smad-dependent expression. May also hydrolyze PtdIns(1, 3, 4, 5)P4, and could thus affect the levels of the higher inositol polyphosphates like InsP6.
Nucleotide accession_num	NM_001017915.2
Protein accession_num	NP_001017915.1
Protein name	Phosphatidylinositol 3, 4, 5-trisphosphate 5-phosphatase 1
Kit Components	Component: Amount INPP5D Microplate: 96 Wells (12 x 8 Well strips)INPP5D Lyophilized Standard: 2100X INPP5D HRP-Detector Anitbody: 1 x 120 uL100X Avidin-HRP Conjugate: 1 x 120 uLStandard Diluent: 1 x 20 mLDetector Antibody Diluent: 1 x 12 mLConjugate Diluent: 1 x 12 mL30X Wash Buffer: 1 x 20 mLTMB Substrate: 1 x 2 mLStop Solution: 1 x 20 mL
Kit detection	Colorimetric
Kit duration	3h
Kit principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Inositol Polyphosphate-5-Phosphatase 145kDa (INPP5D). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Inositol Polyphosphate-5-Phosphatase 145kDa (INPP5D). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Inositol Polyphosphate-5-Phosphatase 145kDa (INPP5D), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Inositol Polyphosphate-5-Phosphatase 145kDa (INPP5D) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Inositol Polyphosphate-5-Phosphatase 145kDa (INPP5D) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Inositol Polyphosphate-5-Phosphatase 145kDa (INPP5D) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Serum, plasma, tissue homogenates and other biological fluids

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OKCD09350.pdf

OKCD09350-datasheet.pdf

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Menge: 96 Wells

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