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ZFAND6 ELISA Kit (Human)

ArtNr OKCD09377
Hersteller AVIVA Systems Biology
Menge 96 Wells
Kategorie
Typ Elisa-Kit
Applikationen ELISA
Specific against Human (Homo sapiens)
Sensitivity < 0.056ng/mL
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias AN1-type zinc finger protein 6;Associated with PRK1 protein;AWP1;protein associated with PRK1;ZA20D3;ZFAND5B;Zinc finger A20 domain-containing protein 3;zinc finger,A20 domain containing 3;zinc finger,AN1-type domain 6.
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
ELISA Kit
Manufacturer - Applications
Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category
Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature
Wet Ice
Gene symbol
ZFAND6
Gene Fullname
zinc finger AN1-type containing 6
Reconstitution and storage
2°C to 8°C|-20°C
Description of target
Involved in regulation of TNF-alpha induced NF-kappa-B activation and apoptosis. Involved in modulation of 'Lys-48'-linked polyubiquitination status of TRAF2 and decreases association of TRAF2 with RIPK1. Required for PTS1 target sequence-dependent protein import into peroxisomes and PEX5 stability; may cooperate with PEX6. In vitro involved in PEX5 export from the cytosol to peroxisomes (By similarity).
Nucleotide accession_num
NM_001242911.1
Protein accession_num
NP_001229840.1
Protein name
AN1-type zinc finger protein 6
Kit Components
Component: Amount
ZFAND6 Microplate: 96 Wells (12 x 8 Well strips)ZFAND6 Lyophilized Standard: 2100X ZFAND6 HRP-Detector Anitbody: 1 x 120 uL100X Avidin-HRP Conjugate: 1 x 120 uLStandard Diluent: 1 x 20 mLDetector Antibody Diluent: 1 x 12 mLConjugate Diluent: 1 x 12 mL30X Wash Buffer: 1 x 20 mLTMB Substrate: 1 x 9 mLStop Solution: 1 x 6 mL
Kit detection
Colorimetric
Kit duration
3h
Kit principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Zinc Finger, AN1-Type Domain Protein 6 (ZFAND6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Zinc Finger, AN1-Type Domain Protein 6 (ZFAND6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Zinc Finger, AN1-Type Domain Protein 6 (ZFAND6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Zinc Finger, AN1-Type Domain Protein 6 (ZFAND6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Kit reproducibility
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Zinc Finger, AN1-Type Domain Protein 6 (ZFAND6) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Zinc Finger, AN1-Type Domain Protein 6 (ZFAND6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type
Tissue homogenates and other biological fluids.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96 Wells
Lieferbar: In stock
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