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COPR5 ELISA Kit (Human)

COPR5 ELISA Kit (Human)

ArtNr	OKCD09483
Hersteller	AVIVA Systems Biology
Menge	96 Wells
Kategorie	Oncology By Cell Type Carcinoma Hematology Lymphoma Myeloma Sarcoma ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Colorectal Cancer Testicular & Prostate Cancer Immunostaining
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	< 0.055ng/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	C17orf79;cooperator of PRMT5;coordinator of PRMT5 and differentiation stimulator;coordinator of PRMT5,differentiation stimulator;COPR5;HSA272196;Protein TTP1;TTP1.
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Type	ELISA Kit
Manufacturer - Applications	Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	COPRS
Gene Fullname	coordinator of PRMT5 and differentiation stimulator
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	Histone-binding protein required for histone H4 methyltransferase activity of PRMT5. Specifically required for histone H4 'Arg-3' methylation mediated by PRMT5, but not histone H3 'Arg-8' methylation, suggesting that it modulates the substrate specificity of PRMT5. Specifically interacts with the N-terminus of histone H4 but not with histone H3, suggesting that it acts by promoting the association between histone H4 and PRMT5. Involved in CCNE1 promoter repression. Plays a role in muscle cell differentiation by modulating the recruitment of PRMT5 to the promoter of genes involved in the coordination between cell cycle exit and muscle differentiation (By similarity).
Nucleotide accession_num	NM_018405.3
Protein accession_num	NP_060875.2
Protein name	Coordinator of PRMT5 and differentiation stimulator
Kit Components	Component: Amount COPR5 Microplate: 96 Wells (12 x 8 Well strips)COPR5 Lyophilized Standard: 2100X COPR5 HRP-Detector Anitbody: 1 x 120 uL100X Avidin-HRP Conjugate: 1 x 120 uLStandard Diluent: 1 x 20 mLDetector Antibody Diluent: 1 x 12 mLConjugate Diluent: 1 x 12 mL30X Wash Buffer: 1 x 20 mLTMB Substrate: 1 x 9 mLStop Solution: 1 x 6 mL
Kit detection	Colorimetric
Kit duration	3h
Kit principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Coordinator Of PRMT5 And Differentiation Stimulator (COPRS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Coordinator Of PRMT5 And Differentiation Stimulator (COPRS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Coordinator Of PRMT5 And Differentiation Stimulator (COPRS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Coordinator Of PRMT5 And Differentiation Stimulator (COPRS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Coordinator Of PRMT5 And Differentiation Stimulator (COPRS) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Coordinator Of PRMT5 And Differentiation Stimulator (COPRS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Tissue homogenates and other biological fluids.

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OKCD09483.pdf

OKCD09483-datasheet.pdf

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Menge: 96 Wells

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Lieferung vsl. bis 25.12.2025

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