IL-12 High Sensitivity ELISA Kit (Human)

Root Catalog/Products/Kit

25 kDa

interleukin 12A

Store as indicated in product manual.

NM_000882.3

interleukin-12 subunit alpha precursor

< 4 Hours

Aviva Systems Biology IL-12p70 High Sensitivity ELISA Kit (Human) (OKDB00043) is designed for the highly sensitive in-vitro quantitative measurement of recombinant or natural IL-12p70 in human serum, plasma buffered solutions or cell culture medium. The kit is based on standard sandwich enzyme-linked immuno-sorbent assay methods along with an additional detection process employing a polymerization step to amplify the number of detector bound HRP molecules. A monoclonal antibody specific for IL-12p70 has been pre-coated into wells of a 96-well plate. Standards or test samples are added to the wells and incubated. After washing, a biotinylated detector antibody specific for IL-12p70 is added, incubated and followed by washing. Streptavidin-HRP conjugate is then added, incubated and unbound conjugate is washed away. Amplification is achieved by adding a Biotin-Tyramine reagent, incubating and washing, followed by a second incubation with Streptavidin-HRP conjugate. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample IL-12p70 captured in well.

The mean intra-assay and mean inter-assay coefficient of variation has been calculated to be 7.9% and 9.5% respectively.