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OSTN ELISA Kit (Human)

ArtNr OKDD00447
Hersteller AVIVA Systems Biology
Menge 96 tests
Kategorie
Typ Elisa-Kit
Applikationen ELISA
Specific against Human (Homo sapiens)
Sensitivity < 0.058 ng/mL
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias MUSCLIN;osteocrin.
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
ELISA Kit
Manufacturer - Category
Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature
Wet Ice
Molecular Weight
15 kDa
Gene symbol
OSTN
Gene Fullname
osteocrin
Protein size
133
Reconstitution and storage
Store as indicated in product manual.
Description of target
Hormone that acts as a regulator of dendritic growth in the developing cerebral cortex in response to sensory experience (PubMed:27830782). Induced in the brain following membrane depolarization and inhibits dendritic branching in neurons of the developing cortex (PubMed:27830782). Probably acts by binding to natriuretic peptide receptor NPR3/NPR-C, thereby preventing binding between NPR3/NPR-C and natriuretic peptides, leading to increase cGMP production (By similarity).
Nucleotide accession_num
NM_198184.1
Protein accession_num
NP_937827.1
Protein name
Osteocrin
Manufacturer - Specificity
This assay has high sensitivity and excellent specificity for detection of OSTN.No significant cross-reactivity or interference between OSTN and analogues was observed.
Assay info
Quantitative Sandwich ELISA
Kit duration
~4 Hours
Kit principle
Aviva Systems Biology OSTN ELISA Kit (Human) (OKDD00447) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for OSTN has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for OSTN is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample OSTN captured in well.
Kit reproducibility

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level OSTN were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level OSTN were tested on 3 different plates, 8 replicates in each plate.

Intra-Assay: CV<10%

Inter-Assay: CV<12%

Sample Type
Serum, Plasma, Tissue homogenates

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96 tests
Lieferbar: In stock
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