VEGFR2 ELISA Kit (Mouse)

ELISA Kit

Wet Ice

Kdr

1345

NM_010612.2

Kinase insert domain protein receptor

Quantitative Sandwich ELISA

Aviva Systems Biology VEGFR2 ELISA Kit (Mouse) (OKDD00631) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for VEGFR2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for VEGFR2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample VEGFR2 captured in well.

Serum, plasma, tissue homogenates