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IL8Ra ELISA Kit (Rat)

ArtNr OKDD00639
Hersteller AVIVA Systems Biology
Menge 96 tests
Kategorie
Typ Elisa-Kit
Applikationen ELISA
Specific against Rat (Rattus norvegicus)
Sensitivity <0.056 ng/mL
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias chemokine (C-X-C motif) receptor 1;C-X-C chemokine receptor type 1;CXCR-1;CXC-R1;high affinity interleukin-8 receptor A;IL-8R A;Il8ra;interleukin 8 receptor,alpha.
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
ELISA Kit
Manufacturer - Category
Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature
Wet Ice
Molecular Weight
40 kDa
Gene symbol
Cxcr1
Gene Fullname
C-X-C motif chemokine receptor 1
Protein size
349
Reconstitution and storage
Store as indicated in product manual.
Description of target
Receptor to interleukin-8, which is a powerful neutrophils chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activate a phosphatidylinositol-calcium second messenger system (By similarity).
Nucleotide accession_num
NM_019310.1
Protein accession_num
NP_062183.1 
Protein name
C-X-C chemokine receptor type 1
Manufacturer - Specificity
This assay has high sensitivity and excellent specificity for detection of IL8Ra.No significant cross-reactivity or interference between IL8Ra and analogues was observed.
Assay info
Quantitative Sandwich ELISA
Kit duration
~4 Hours
Kit principle
Aviva Systems Biology IL8Ra ELISA Kit (Rat) (OKDD00639) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for IL8Ra has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IL8Ra is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample IL8Ra captured in well.
Kit reproducibility

Intra-assay Precision: 3 samples with low, middle and high level analyte were measured with 20 replicates on one plate, respectively.

Inter-assay Precision: 3 samples with low, middle and high level analyte were measured across 3 different plates, with 8 replicates per plate.

Mean Intra-Assay: CV<10%

Mean Inter-Assay: CV<12%

Sample Type
Serum, plasma, tissue homogenates

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96 tests
Lieferbar: In stock
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