AMSH ELISA Kit (Human)

Enzyme-linked Immunosorbent assay-Competitive

Wet Ice

Store as indicated in product manual.

STAM-binding protein

Assay Methodology: Quantitative Competitive ELISA

Aviva Systems Biology AMSH ELISA Kit (Human) (OKEH00326) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for AMSH has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for AMSH is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample AMSH captured in well.

Mean Intra-Assay Precision - < 4.2%, n = 20
Mean Inter-Assay Precision - < 7.6%, n = 20