VIP ELISA Kit (Human)

ELISA Kit

Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit

19 kDa

vasoactive intestinal peptide

Store as indicated in product manual.

NM_003381.3

P01282

Assay Methodology: Quantitative Sandwich ELISA

Aviva Systems Biology VIP ELISA Kit (Human) (OKEH00406) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human VIP antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Human VIP and incubated. The Human VIP found in the sample or standards competes with the biotinylated Human VIP for limited binding sites on the immobilized anti-Human VIP antibody. Excess unbound biotinylated Human VIP and sample or standard human VIP is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Human VIP captured in the well and inversely proportional to the amount of human VIP which was contained in the sample or standard.

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids