ICTP ELISA Kit (Human)

Enzyme-linked Immunosorbent assay-Competitive

Wet Ice

Store as indicated in product manual.

Cross-linked C-terminal telopeptide of Collagen alpha-1(I) chain

Assay Methodology: Quantitative Competitive ELISA

Aviva Systems Biology ICTP ELISA Kit (Human) (OKEH00680) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human ICTP antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated ICTP and incubated. The ICTP found in the sample or standards competes with the biotinylated ICTP for limited binding sites on the immobilized anti-human ICTP antibody. Excess unbound biotinylated ICTP and sample or standard ICTP is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated ICTP captured in the well and inversely proportional to the amount of ICTP which was contained in the sample or standard.

Mean Intra-assay CV%: < 4.4% (n = 20)
Mean Inter-assay CV%: < 8.1% (n = 20)