CTCFL ELISA Kit (Human)

ELISA Kit

Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit

75 kDa

CCCTC-binding factor like

Store as indicated in product manual.

NM_001269040.1

Q8NI51

Assay Methodology: Quantitative Competitive ELISA

Aviva Systems Biology CTCFL ELISA Kit (Human) (OKEH01659) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human CTCFL antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated CTCFL and incubated. The CTCFL found in the sample or standards competes with the biotinylated CTCFL for limited binding sites on the immobilized anti-human CTCFL antibody. Excess unbound biotinylated CTCFL and sample or standard CTCFL is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated CTCFL captured in the well and inversely proportional to the amount of CTCFL which was contained in the sample or standard.

Mean Intra-assay CV%: < 6.3% (n = 20)
Mean Inter-assay CV%: < 11.5% (n = 20)