PIK3CA ELISA Kit (Mouse)

ELISA Kit

Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit

124 kDa

phosphatidylinositol-4, 5-bisphosphate 3-kinase catalytic subunit alpha

Store as indicated in product manual.

NM_008839.2

P42337

Assay Methodology: Quantitative Sandwich ELISA

Aviva Systems Biology PIK3CA ELISA Kit (Mouse) (OKEH02352) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PIK3CA has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PIK3CA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PIK3CA captured in well.

Mean Intra-assay CV%: < 6.2% (n = 20)
Mean Inter-assay CV%: < 11.3% (n = 20)