HPGDS ELISA Kit (Human)

ELISA Kit

Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit

23 kDa

hematopoietic prostaglandin D synthase

Store as indicated in product manual.

NM_014485.2

O60760

Assay Methodology: Quantitative Sandwich ELISA

Aviva Systems Biology HPGDS ELISA Kit (Human) (OKEH02472) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human HPGDS antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated HPGDS and incubated. The HPGDS found in the sample or standards competes with the biotinylated HPGDS for limited binding sites on the immobilized anti-Human HPGDS antibody. Excess unbound biotinylated HPGDS and sample or standard HPGDS is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated HPGDS captured in the well and inversely proportional to the amount of HPGDS which was contained in the sample or standard.

Mean Intra-assay CV%: < 7.4% (n = 20)
Mean Inter-assay CV%: < 9.3% (n = 20)