ATR ELISA Kit (Mouse)

ELISA Kit

Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit

301 kDa

ataxia telangiectasia and Rad3 related

Store as indicated in product manual.

NM_019864.1

Q9JKK8

Assay Methodology: Quantitative Competitive ELISA

Aviva Systems Biology ATR ELISA Kit (Mouse) (OKEH02515) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Mouse ATR antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated ATR and incubated. The ATR found in the sample or standards competes with the biotinylated ATR for limited binding sites on the immobilized anti-Mouse ATR antibody. Excess unbound biotinylated ATR and sample or standard ATR is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated ATR captured in the well and inversely proportional to the amount of ATR which was contained in the sample or standard.

Mean Intra-assay CV%: < 8.4% (n = 20)
Mean Inter-assay CV%: < 11.1% (n = 20)